Journal: Medicine International

Article Title: Ovarian fibroma torsion mimicking solid malignant tumors of the ovary: A case report and mini-review of the literature

doi: 10.3892/mi.2025.280

Figure Lengend Snippet: Immunohistochemical images of twisted ovarian fibroma: (A) Reticulin positivity (magnification, x100), (B) WT1 positivity (magnification, x400), (C) inhibin focal positivity (magnification, x400), (D) calretinin negativity (magnification, x40).

Article Snippet: The block peroxide kit (Bond; Leica Biosystems) was subsequently used for 5 min. For WT1, the anti-WT1 antibody (cat. no. AMAb91842, Atlas Antibodies) was used at a 1:1,200 dilution.

Techniques: Immunohistochemical staining

Journal: International Journal of Molecular Sciences

Article Title: Identification of Common Genes Regulated by ER Stress During the Development of Diabetic Nephropathy Based on Human Transcriptome Datasets and an In Vivo Mouse Model

doi: 10.3390/ijms27052491

Figure Lengend Snippet: 4-PBA reduced podocyte loss and tubular interstitial fibrosis in DN. Kidney sections were processed for PAS staining (glomerular area in ( A ) and tubular area in ( B )), WT1 immunohistochemistry ( C ), and Picro-Sirius Red staining ( D ) ( n = 3); representative images are shown (black arrows indicate damaged renal areas). Relative mRNA levels of Nphs-1 and Nphs-2 (specific markers for podocyte loss), as well as α-SMA and collagen I (fibrogenic markers), were assessed by real-time PCR analysis ( E ) ( n = 3–5). Relative mRNA expression was normalized to that of GAPDH ( n = 3–5). Data are presented as the mean ± SEM. One-way ANOVA was used for statistical analysis, followed by Bonferroni’s multiple comparisons. * p < 0.05, ** p < 0.01 vs. control mice; and # p < 0.05, ## p < 0.01 vs. DN mice. Scale bar, 100 µm.

Article Snippet: The sections were blocked in 10% normal horse serum and incubated with a primary antibody against CD68 (Cluster of Differentiation 68, Abcam) or Wilms’ Tumor Protein 1 (WT1) from Boster Biological Technology (Pleasanton, CA, USA), overnight at 4 °C.

Techniques: Staining, Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Control

Journal: Molecular Metabolism

Article Title: P2Y2R contributes to the development of diabetic nephropathy by inhibiting autophagy response

doi: 10.1016/j.molmet.2020.101089

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: Wilms Tumor-1 (WT1) , Boster Biological Technology , #M00199-1 , Rabbit , 1 μg/ml (IHC).

Techniques:

Journal: Molecular Metabolism

Article Title: P2Y2R contributes to the development of diabetic nephropathy by inhibiting autophagy response

doi: 10.1016/j.molmet.2020.101089

Figure Lengend Snippet: P2Y2R deficiency protects against podocyte loss and glomerular injury in DN. (A) Relative mRNA levels of Nphs1 (nephrin) and Nphs2 (podocin) were determined by real-time PCR analysis (n = 3–5). (B) Localisation of WT1, a podocyte marker, was analysed by immunohistochemistry, and representative images are shown. The number of stained podocytes per glomeruli was counted by using ImageJ software (n = 3). (C) Kidney sections were stained with PAS staining and glomerular morphological changes were scored as described in the method section (n = 3). Data are presented as mean ± SEM. One-way ANOVA was used for statistical analysis followed by Bonferroni's multiple comparisons test. ∗∗∗p < 0.001 vs WT control mice; and ### p < 0.001 vs WT DN mice. Scale bar, 50 μm.

Article Snippet: Wilms Tumor-1 (WT1) , Boster Biological Technology , #M00199-1 , Rabbit , 1 μg/ml (IHC).

Techniques: Real-time Polymerase Chain Reaction, Marker, Immunohistochemistry, Staining, Software, Control

Journal: International Journal of Biological Sciences

Article Title: APT1-derived depalmitoylation of CD36 alleviates diabetes-induced lipotoxicity in podocytes

doi: 10.7150/ijbs.109220

Figure Lengend Snippet: Construction of db/db model to evaluate changes in renal pathology lipid metabolism. Two groups of twenty-week-old mice were analyzed: db/m (n=6) and db/db (n=6). A-B. Representative PAS staining of glomeruli in each group, and the semiquantitative analysis of mesangial area ratio. (**<0.01, scale bar: 50 μm). C-G. Biochemical examination: (C) UACR, (D) serum creatinine, (E) blood glucose, (F) serum cholesterol, (G) serum triglycerides. (**<0.01). H. Representative transmission electron microscopy (TEM) images of podocyte foot processes and glomerular basement membrane (GBM) in each group. (**<0.01, scale bar: 10 μm). I-J. Representative immunofluorescence double staining of BODIPY and WT1 in the glomeruli of db/m and db/db mice, and semiquantitative analysis. (**<0.01, scale bar:50μm). K-L. Oil Red O staining of lipid droplets (LDs) in the glomeruli of db/m and db/db mice, and semiquantitative analysis. (**<0.01, scale bar: 40 μm).

Article Snippet: Frozen kidney sections were fixed, blocked, and then stained with WT1 antibody (BM4216, Boster, China) for 1 h at 37°C, subsequently incubated in the presence of secondary antibody and BODIPY dye, and finally covered with DAPI.

Techniques: Staining, Transmission Assay, Electron Microscopy, Membrane, Immunofluorescence, Double Staining